Monday 26 November 2012

36 ATP created through Cellular Respiration

If you are a biology study you should know the equation for cellular respiration is C6H12O6  + 6 O2  → 6 CO2  + 6 H2O + 36 ATP
Just because biology is super amusing and fun, let's count the ATP made in cellular respiration.

  • 2 in glycolysis
  • 2 in Krebs cycle
  • 34 in the electron transport system
But wait that's a total of 38 ATP! But the cellular respiration equation can't be wrong, so where did the extra 2 ATP go?  If we can recall the ETC requires NADH to donate the first 2 electrons, and where do we get these electrons?  2 are from pyruvate oxidation, 6 are from the Krebs cycle, and the last 2 are from glycolysis.  However, we know that the ETC takes place in the mitochondria and 8 of the NADH are already in the mitochondria, except for the 2 NADH from  glycolysis which are still in the cytoplasm.  Thus, to transport the 2 NADH from the cytoplasm into the mitochondria 2 ATP are required.  Therefore, the net equation for cellular respiration still has a total of 36 ATP.

Friday 2 November 2012

Liver Enzyme Lab Results

Our lab was to test the effect of limiting the substrate (H2O2) on the rate of reaction.  Here are the results:

[H2O2] + [H2O]  (mL/s)       Time (s)                      Gas Collected (mL)                Rate of Reaction (mL/s)

3mL + 0mL                              65                              250                                          3.85
2.5mL + 0.5mL                        27                              250                                          9.26             
2mL + 1mL                              40                              250                                          6.25
1.5mL + 1.5 mL                       47                              250                                          5.32
1mL + 2mL                              60                              250                                          4.17        
0.5mL + 2.5 mL                       20                              250                                          12.5